Rewriting the Metabolic Blueprint: Advances in Pathway Diversification in Microorganisms

Hossain GS, Nadarajan SP, Zhang L, Ng TK, Foo JL, Ling H, Choi WJ, Chang MW

Frontiers in Microbiology 9:155 (2018)

Engineered commensal microbes for dietmediated colorectal-cancer chemoprevention

Ho CL, Tan HQ, Chua KJ, Kang A, Lim KH, Ling KL, Yew WS, Lee YS, Thiery JP, Chang MW

Nature Biomedical Engineering 2:27–37 (2018)


One-Pot Enantioselective Synthesis of d-Phenylglycines from Racemic Mandelic Acids, Styrenes, or Biobased l-Phenylalanine via Cascade Biocatalysis

Zhou Y, Wu S, Li Z

Advanced Synthesis and Catalysis 359(24):4305–4316 (2017)

Microscale Bioreactors for in situ characterization of GI epithelial cell physiology

Costello C, Philipson M, Hartmanis L, Kwasnica M, Chen V, Hackam D, Chang MW, Bentley W, March J

Scientific Reports 7:12515 (2017)

Autoinducer sensing microarrays by reporter bacteria encapsulated in hybrid supramolecular-polysaccharide hydrogels

Li P, Dou XO, Müller M, Feng C, Chang MW, Frettlöh M, Schönherr H

Macromolecular Bioscience 1700176:8-16 (2017)

Encapsulation of Autoinducer Sensing Reporter Bacteria in Reinforced Alginate-based Microbeads

Li P, Müller M, Chang MW, Frettlöh M, Schönherr H

ACS Appl Mater Interfaces 9 (27):22321–22331 (2017)

Engineering a riboswitch-based genetic platform for the self-directed evolution of acid-tolerant phenotypes

Pham HL, Wong A, Chua N, Teo WS, Yew WS, and Chang MW

Nature Communications 8:411 (2017)

Isolated Reporter Bacteria in Supramolecular Hydrogel Microwell Arrays

Li P, Dou XQ, Feng C, Müller M, Chang MW, Frettlöh M, Schönherr H

Langmuir 33 (31):7799–7809(2017)

Designer probiotics for the prevention and treatment of human diseases

Chua KJ, Kwok WC, Aggarwal N, Sun T, Chang MW

Current Opinion in Chemical Biology 40:8-16 (2017)

Synthetic gene design using Codon Optimization On-Line (COOL)

Yu K, Ang KS, Lee DY

Methods in Molecular Biology 1472:13-34 (2017)

Engineered probiotic Escherichia coli can eliminate and prevent Pseudomonas aeruginosa infection in animal models

Hwang IY, Koh E, Wong CK, March JC, Bentley WE, Lee YS, Chang MW

Nature Communications 8:15028 (2017)

Applying the Design-Build-Test paradigm in microbiome engineering

Pham HL, Ho CL, Wong A, Lee YS, Chang MW

Current Opinion in Biotechnology 48:85-93 (2017)

The fungal mycobiome and interaction with gut bacteria in the host

Sam QH, Chang MW, Chai LYA

International Journal of Molecular Sciences 18:330-341 (2017)

Microbiome engineering: current applications and its future

Foo JL, Ling H, Lee YS, Chang MW

Biotechnology Journal 12:e1600099 (2017)


Highly regio- and enantioselective multiple oxy- and amino-functionalizations of alkenes by modular cascade biocatalysis

Wu S, Zhou Y, Wang T, Too HP, Wang DIC, Li Z

Nature Communications 7:11917 (2016)

Cascade biocatalysis for sustainable asymmetric synthesis: from biobased I-phenylalanine to high-value chiral chemicals

Zhou Y, Wu S, Li Z

Angewandte Chemie 55:11647-11650 (2016)

Multi-omics data driven analysis establishes reference codon biases for synthetic gene design in microbial and mammalian cells

Ang KS, Kyriakopoulos S., Li W, Lee DY

Methods 102:26-35 (2016)

Codon optimization of Saccharomyces cerevisiae mating factor alpha prepro-leader to improve recombinant protein production in Pichia pastoris

Ahn J, Jang MJ, Ang KS, Lee H, Choi ES, Lee DY

Biotechnology Letters 38:2137-2143 (2016)

A genetic algorithm-based approach for pre-processing metabolomics and lipidomics LC-MS data

Yeo HC, Chung BKS, Chong W, Chin JX, Ang KS, Lakshmanan M, Ho YS, Lee DY

Metabolomics 12: 5 (2016)

Transcriptomics-based strain optimization tool for designing secondary metabolite overproducing strains of Streptomyces coelicolor

Kim M, Yi JS, Lakshmanan M, Lee DY, Kim BG

Biotechnology and Bioengineering 113:651-660 (2016)

Coordinate regulation of metabolite glycosylation and stress hormone biosynthesis by TT8 in Arabidopsis

Rai A, Umashankar S, Rai M, Lim BK, Aow JSB, Swarup S

Plant Physiology 171:2499-2515 (2016)

Flagellin FliC phosphorylation affects type 2 protease secretion and biofilm dispersal in Pseudomonas aeruginosa PAO1

Suriyanarayanan T, Periasamy S, Lin MH, Ishihama Y, Swarup S

PLoS One 11:e0164155 (2016)

Reprogrammable microbial cell-based therapeutics against antibiotic-resistant bacteria

Hwang IY, Koh E, Kim HR, Chang MW

Drug Resistance Updates 27:59-71 (2016)

Anti-virulent disruption of pathogenic biofilms using engineered quorum-quenching lactonases

Tay SB, Chow JY, Go M, Yew WS

Journal of Visualized Experiments 107:e53243 (2016)

Exploiting the biosynthetic potential of type III polyketide synthases

Lim YP, Go M, Yew WS

Molecules 21: 53-61 (2016)

A two-layer gene circuit for decoupling cell growth from metabolite production

Lo T, Chng SH, Teo WS, Cho HS, Chang MW

Cell Systems 3:133-143 (2016)

Metabolic engineering of Saccharomyces cerevisiae for the overproduction of short branched-chain fatty acids

Yu A, Pratomo NK, Foo JL, Leong SSJ, Chang MW

Metabolic Engineering 34:36-43 (2016)

Whole-cell biocatalytic and de novo production of alkanes from free fatty acids in Saccharomyces cerevisiae

Foo JL, Susanto AV, Keasling JD, Leong SSJ, Chang MW

Biotechnology and Bioengineering 114:232-237 (2016)

Genetic engineering of an unconventional yeast for renewable biofuel and biochemical production

Yu AQ, Pratomo N, Ng TK, Ling H, Cho HS, Leong SS, Chang MW

Journal of Visualized Experiments 115:e54371 (2016)

Synthetic biology in Asia: New kid on the block

Chang MW

ACS Synthetic Biology 5: 1182-1183 (2016)

Genome-scale metabolic modeling in silico analysis of lipid accumulating yeast Candida tropicalis for dicarboxylic acid production

Mishra P, Park GY, Lakshmanan M, Lee HS, Lee H, Chang MW, Ching CB, Ahn J, Lee DY

Biotechnology and Bioengineering 113: 1993-2004 (2016)


SynLinker: an integrated system for designing linkers and synthetic fusion proteins

Liu CC, Chin JX, Lee DY

Bioinformatics 22:3700-3702 (2015)

Synthetic fusion protein design and applications

Yu K, Liu CC, Kim BG, Lee DY

Biotechnology Advances 33:155-164 (2015)

Effect of linker flexibility and length on the functionality of a cytotoxic engineered antibody fragment

Klement M, Liu CC, Loo BLW, Choo ABH, Ow DSW, Lee DY

Journal of Biotechnology 199: 90-97 (2015)

In silico model-driven cofactor engineering strategies for improving the overall NADP(H) bioavailability in microbial cell factories

Lakshmanan M, Yu K, Koduru L, Lee DY

Journal of Industrial Microbiology and Biotechnology 42: 1401-1414 (2015)

Epoxide hydrolase-lasalocid A structure provides mechanistic insight into polyether natural product biosynthesis

Wong FT, Jotta K, Chen X, Fang M, Watanabe K, Kim CY

Journal of the American Chemical Society 137: 86-89 (2015)

Combinatorial metabolic engineering of Saccharomyces cerevisiae for terminal alkene production

Chen BB, Lee DY, Chang MW

Metabolic Engineering 31:53-61 (2015)

Bacterial XylRs and synthetic promoters function as genetically encoded xylose biosensors in Saccharomyces cerevisiae

Teo WS, Chang MW

Biotechnology Journal 10:315-322 (2015)

Matrix-immobilized yeast for large-scale production of recombinant human lactoferrin

Ho CL, Hwang IY, Loh K, Chang MW

Med Chem Comm 6:486-491 (2015)

Engineering transcription factors to improve against alkane biofuels in Saccharomyces cerevisiae

Ling H, Pratomo NK, Teo WS, Liu R, Leong SSJ, Chang MW

Biotechnology for Biofuels 8:231-242 (2015)

Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid short- and branched-chain alkyl esters biodiesel

Teo WS, Yu A, Chang MW

Biotechnology for Biofuels 8:177-185 (2015)

Synthetic polyketide enzymology: platform for biosynthesis of antimicrobial polyketides

Go M, Wongsantichon J, Cheung, VWN, Chow JY, Robinson RC, Yew WS

ACS Catalysis 5:4033-4042 (2015)

Engineered strains enhance gold biorecovery from electronic scrap

Natarajan G, Tay SB, Yew WS, Ting YP

Minerals Engineering 75:32-37 (2015)

Engineered Saccharomyces cerevisiae to produce odd chain-length fatty alcohols

Jin Z, Wong A, Foo JL, Ng Joey, Cao YX, Chang MW, Yuan YJ

Biotechnology and Bioengineering 113:842-851 (2015)


Production of fatty acid-derived valuable chemicals in synthetic microbes

Yu AQ, Pratomo Juwono NK, Leong SSJ, Chang MW

Frontiers in Bioengineering and Biotechnology 2:78-89 (2014)

Microbial tolerance engineering toward biochemical production: from lignocellulose to products

Ling H, Teo W, Chen B, Leong SSJ, Chang MW

Current Opinion in Biotechnology 29:99-106 (2014)

Site specific immobilization of a potent antimicrobial peptide onto silicone catheters: evaluation against urinary tract infection pathogens

Mishra B, Basu A, Chua RRY, Saravanan R, Tambyah PA, Ho B, Chang MW, Leong SSJ

Journal of Materials Chemistry 2:1706-1716 (2014)

Therapeutic microbes for infectious disease

Wong CK, Tan MH, Rasouliha B, Hwang IY, Ling H, Poh CL and Chang MW

Methods in Molecular Biology 1151:117-133 (2014)

Identification of polyketide inhibitors targeting 3-dehydroquinate dehydratase in the shikimate pathway of Enterococcus faelis

Cheung VWN, Xue B, Valladares MH, Go M, Tung A, Aguda AH, Robinson RC, Yew WS

PLoS ONE 9:e103598 (2014)

Glycine decarboxylase is an unusual amino acid decarboxylase involved in tumorigenesis

Go M, Zhang WC, Lim B, Yew WS

Biochemistry 53: 947-956 (2014)

Disruption of Biofilm Formation by the Human Pathogen Acinetobacter baumannii using engineered quorum-quenching Lactonases

Chow JY, Yang Y, Tay SB, Chua KL, Yew WS

Antimicrobial Agents and Chemotherapy 58: 1802-1805 (2014)

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