Flagship Research Programs

At SynCTI, we have 3 major flagship research programs namely Cells by Design, Industrial Biosolutions and Microbiome Based Solutions, where our devoted researchers are developing engineered biological systems for a solution-driven approach in key areas such as healthcare, energy and environment. This is powered by our top-notch state of the art NUS BioFoundry that aids in accelerating our research to mould biosystems to our advantage.

Cells by Design

Industrial Biosolution

Microbiome-based Solutions

Featured Research

Blue Light Magic

Poh Lab

Blue Light Magic

Bacterial motility is key to many cellular activities like migration and biofilm formation, and exhibit distinct patterns to navigate in a natural environment controlled by chemotaxis. Regulating bacterial patterning through nonchemical based methods such as blue light using principles of optogenetics has potential applications in biomaterial creation, drug delivery, etc. Poh Lab members have created synthetic genetic circuits which can be further regulated by exposure to blue light leading to controlled directional motility and spatial organization of cell population with a specific complex pattern that can be maintained for a longer duration.
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Harvesting baker’s yeast for ageing-related therapeutics

Yew Lab

Harvesting baker’s yeast for ageing-related therapeutics

A class of medicine used in the treatment of neuro-cognitive diseases and other neurological ailments (migraines, headaches, etc) are currently obtained from extracts of the ergot fungus. However, continued cultivation of the ergot fungus for medicine is not sustainable as industrial agriculture is one of the largest contributors to carbon emissions worldwide. o meet the global demand for such medication, between 10-15 tons of D-lysergic acid (DLA), an ingredient used in producing the medicine, are produced each year.

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Press Release

Publications

2022

The transformation of our food system using cellular agriculture: What lies ahead and who will lead it?
Smith DJ, Helmy M, Lindley ND, Selvarajoo K
Trends in Food Science & Technology (2022) In press

Potential use of microbial engineering in single cell protein production
Balaji B, Ling H, Choi WJ, Chang MW
Current Opinion in Biotechnology (2022) In press

Design and fabrication of field-deployable microbial biosensing device
Pahm HL, Ling H, Chang MW
Current Opinion in Biotechnology 76: 102731 (2022)

Single 3′-exonuclease-based multifragment DNA assembly method (SENAX)
Dao VL, Chan S, Zhang J, Ngo RKJ, Poh CL
Scientific Reports 12: 4004 (2022)

Structure-guided engineering of prenyltransferase NphB for high-yield and regioselective cannabinoid production
Lim KJH, Hartono YD, Xue B, Go MK, Fan H, Yew WS
ACS Catalysis 12: 4628-4639 (2022)

Living building blocks
Wun KS, Hwang IY, Chang MW
Nature Materials 21 (4): 382-383

Genetic circuit design principles
Yeoh JW, Gomez-Carretero S, Chee WKD, Teh AY, Poh CL
Handbook of Cell Biosensors: 339-381 (2022)

Highly reversible tunable thermal-repressible split-T7 RNA polymerases (thermal-T7RNAPs) for dynamic gene regulation
WKD Chee, Yeoh JW, Dao VL, Poh CL
ACS Synthetic Biology 11 (2): 921-937 (2022)

Transporter-driven engineering of a genetic biosensor for the detection and production of short-branched chain fatty acids in Saccharomyces cerevisiae
Miyake R, Ling H, Foo JL, Fugono N, Chang MW
Frontiers in Bioengineering and Biotechnology 10: 838732 (2022)

Synthetic biology: at the crossroads of genetic engineering and human therapeutics-a Keystone Symposia report
Cable J, Leonard JN, Lu TK, Xie Z, Chang MW, Fernandez LA, Lora JM, Kaufman HL, Quintana FJ, Geiger R, Lesser CF, Lunch JP, Hava DL, Cornish VW, Lee GK, DiAndreth B, Fero M, Srivastava R, Coster TD, Roybal KT, Rackham OJL, Kiani S, Zhu I, Hernandez-Lopez RA, Guo T, Chen WCW
Annals of the New York Academy of Sciences. 1506(1): 98-117 (2021)

Reconstituting the complete biosynthesis of D-lysergic acid in yeast
Wong G, Lim LR, Tan YQ, Go MK, Bell DJ, Freemont PS, Yew WS
Nature Communications 13: 712 (2022)

Thermogenetics: applications come of age
Chee WKD, Yeoh JW, Dao VL, Poh CL
Biotechnology Advances 55: 107907 (2022)

Identifying toggle genes from transcriptome-wide scatter: a new perspective for biological regulation
Giuliani A, Bui TT, Helmy M, Selvarajoo K
Genomics 114(1): 215-228 (2022)

Toward multiplexed optogenetic circuits
Dwijayanti A, Zhang C, Poh CL, Lautier T
Frontiers in Bioengineering and Biotechnology 9: 804563 (2022)

Recent advances in structure, function, and pharmacology of class A lipid GPCRs: opportunities and challenges for drug discovery
Krishna Deepak RNV, Verma RK, Hartono YD, Yew WS, Fan H
Pharmaceuticals 15(1):12 (2022)

Biologically engineered microbes for bioremediation of electronic waste: wayposts, challenges and future directions
Han P, Teo WZ, Yew WS
Engineering Biology 6(1): 23-34 (2022)

Bioproduction of natural phenethyl acetate, phenylacetic acid, ethyl phenylacetate, and phenethyl phenylacetate from renewable feedstock
Sekar BS, Li X, Li Z
ChemSusChem: e202102645 (2022)


2022

The transformation of our food system using cellular agriculture: What lies ahead and who will lead it?
Smith DJ, Helmy M, Lindley ND, Selvarajoo K
Trends in Food Science & Technology (2022) In press

Potential use of microbial engineering in single cell protein production
Balaji B, Ling H, Choi WJ, Chang MW
Current Opinion in Biotechnology (2022) In press

Design and fabrication of field-deployable microbial biosensing device
Pahm HL, Ling H, Chang MW
Current Opinion in Biotechnology 76: 102731 (2022)

Single 3′-exonuclease-based multifragment DNA assembly method (SENAX)
Dao VL, Chan S, Zhang J, Ngo RKJ, Poh CL
Scientific Reports 12: 4004 (2022)

Structure-guided engineering of prenyltransferase NphB for high-yield and regioselective cannabinoid production
Lim KJH, Hartono YD, Xue B, Go MK, Fan H, Yew WS
ACS Catalysis 12: 4628-4639 (2022)

Living building blocks
Wun KS, Hwang IY, Chang MW
Nature Materials 21 (4): 382-383

Genetic circuit design principles
Yeoh JW, Gomez-Carretero S, Chee WKD, Teh AY, Poh CL
Handbook of Cell Biosensors: 339-381 (2022)

Highly reversible tunable thermal-repressible split-T7 RNA polymerases (thermal-T7RNAPs) for dynamic gene regulation
WKD Chee, Yeoh JW, Dao VL, Poh CL
ACS Synthetic Biology 11 (2): 921-937 (2022)

Transporter-driven engineering of a genetic biosensor for the detection and production of short-branched chain fatty acids in Saccharomyces cerevisiae
Miyake R, Ling H, Foo JL, Fugono N, Chang MW
Frontiers in Bioengineering and Biotechnology 10: 838732 (2022)

Synthetic biology: at the crossroads of genetic engineering and human therapeutics-a Keystone Symposia report
Cable J, Leonard JN, Lu TK, Xie Z, Chang MW, Fernandez LA, Lora JM, Kaufman HL, Quintana FJ, Geiger R, Lesser CF, Lunch JP, Hava DL, Cornish VW, Lee GK, DiAndreth B, Fero M, Srivastava R, Coster TD, Roybal KT, Rackham OJL, Kiani S, Zhu I, Hernandez-Lopez RA, Guo T, Chen WCW
Annals of the New York Academy of Sciences. 1506(1): 98-117 (2021)

Reconstituting the complete biosynthesis of D-lysergic acid in yeast
Wong G, Lim LR, Tan YQ, Go MK, Bell DJ, Freemont PS, Yew WS
Nature Communications 13: 712 (2022)

Thermogenetics: applications come of age
Chee WKD, Yeoh JW, Dao VL, Poh CL
Biotechnology Advances 55: 107907 (2022)

Identifying toggle genes from transcriptome-wide scatter: a new perspective for biological regulation
Giuliani A, Bui TT, Helmy M, Selvarajoo K
Genomics 114(1): 215-228 (2022)

Toward multiplexed optogenetic circuits
Dwijayanti A, Zhang C, Poh CL, Lautier T
Frontiers in Bioengineering and Biotechnology 9: 804563 (2022)

Recent advances in structure, function, and pharmacology of class A lipid GPCRs: opportunities and challenges for drug discovery
Krishna Deepak RNV, Verma RK, Hartono YD, Yew WS, Fan H
Pharmaceuticals 15(1):12 (2022)

Biologically engineered microbes for bioremediation of electronic waste: wayposts, challenges and future directions
Han P, Teo WZ, Yew WS
Engineering Biology 6(1): 23-34 (2022)

Bioproduction of natural phenethyl acetate, phenylacetic acid, ethyl phenylacetate, and phenethyl phenylacetate from renewable feedstock
Sekar BS, Li X, Li Z
ChemSusChem: e202102645 (2022)


2021

A newly established virus induced gene silencing via seed imbibition for functional genomics at the early germination stages in cotton
Shi G, Hao M, Tian B, Cao G, Chen W, Zhang Q, Zhang Y, Ling H, Li J, Xie Z, Wei F
Industrial Crops and Products 172:114040 (2021)

Metabolic engineering of microbes for monoterpenoid production
Zhu K, Kong J, Zhao B, Rong L, Liu S, Lu Z, Zhang C, Xiao D, Pushpanathan K, Foo JL, Wong A, Yu A
Biotechnology Advances 53: 107837 (2021)

Driving innovation in biotechnology with private-public partnerships: a Singapore perspective
Lee LH & Vasitvas K
Biotechnology Notes 2: 59-62 (2021)

Scalable workflow for green manufacturing: discovery of bacterial lipases for biodiesel production
Chow JY, Choo KLS, Lim YP, Ling LH, Nguyen GKT, Xue B, Chua NH, Yew WS
ACS Sustainable Chemistry & Engineering 9(40): 13450-13459 (2021)

Hybrid promoter engineering strategies in Yarrowia lipolytica: isoamyl alcohol production as a test study
Zhao Y, Liu S, Lu Z, Zhao B, Wang S, Zhang C, Xiao D, Foo JL, Yu A
Biotechnology for Biofuels 14: 149 (2021)

Simultaneous improvement of limonene production and tolerance in Yarrowia lipolytica through tolerance engineering and evolutionary engineering
Li J, Zhu K, Miao L, Rong L, Zhao Y, Li S, Ma L, Li J, Zhang C, Xiao D, Foo JL, Yu A
ACS Synthetic Biology 10(4): 884-896 (2021)

Biosynthesis of nature-inspired unnatural cannabinoids
Lim KJH, Lim YP, Hartono YD, Go MK, Fan H, Yew WS
Molecules 26(10): 2914 (2021)

Genetically encodable scaffolds for optimizing enzyme function
Tan YQ, Xue B, Yew WS
Molecules 26(5): 1389 (2021)

SynBiopython: an open-source software library for Synthetic biology
Yeoh JW, Swainston N, Vegh P, Zulkower V, Carbonell P, Holowko MB, Peddinti G, Poh CL
Synthetic Biology 6(1): ysab001 (2021)

Structure-based virtual screening of CYP1A1 inhibitors: towards rapid tier-one assessment of potential developmental toxicants
Goh JJN, Behn J, Chong CS, Zhong G, Maurer-Stroh S, Fan H, Loo LH
Archives of Toxicology 95: 3031-3048 (2021)

Ubiquitin ligase SMURF2 interacts with filovirus VP40 and promotes egress of VP40 VLPs
Shepley-McTaggart A, Schwoerer MP, Sagum CA, Bedford MT, Jaladanki CK, Fan H, Cassel J, Harty RN
Viruses 13(2): 288 (2021)

Production of (R)-mandelic acid from styrene, L-phenylalanine, glycerol, or glucose via cascade biotransformationsz
Lukito BR, Wang Z, Sekar BS, Li Z
Bioresources and Bioprocessing 8: 22 (2021)

A high-throughput pipeline for scalable kit-free RNA extraction
Han P, Go MK, Chow JY, Xue B, Lim YP, Crone MA, Storch M, Freemont PS, Yew WS
Scientific Reports 11: 23260 (2021)

GeneCloudOmics: A data analytic cloud platform for high-throughput gene expression analysis
Helmy M, Agrawal R, Soudy M, Bui TT, Selvarajoo K
Frontiers in Bioinformatics 1: 693836 (2021)

Systems biology to understand and regulate human retroviral proinflammatory response
Helmy M, Selvarajoo K
Frontiers in Immunology 12: 7366349 (2021)

The need for integrated systems biology approaches for biotechnological applications
Selvarajoo K
Biotechnology Notes 2: 39-43 (2021)

A novel lipase from Lasiodiplodia theobromae efficiently hydrolyses C8-C10 methyl esters for the preparation of medium-chain triglycerides’ precursors
Ng AMJ, Yang R, Zhang H, Xue B, Yew WS, Nguyen GKT
International Journal of Molecular Sciences 22(19): 10339 (2021)

Heterologous expression of cyanobacterial gas vesicle proteins in Saccharomyces cerevisiae
Jung H, Ling H, Tan YQ, Chua NH, Yew WS, Chang MW.
Biotechnology Journal 9: e2100059 (2021)

Structure of a minimal α-carboxysome-derived shell and its utility in enzyme stabilization
Tan YQ, Ali S, Xue B, Teo WZ, Ling LH, Go MK, Lv H, Robinson RC, Narita A, Yew WS.
Biomacromolecules 22(10): 4095-4109 (2021)

Engineered probiotics modulate the endocannabinoid system
Hwang IY, Kim HR, De Sotto R, Chang MW.
Biotechnology Notes 2: 33-38 (2021)

The divergent immunomodulatory effects of short chain fatty acids and medium chain fatty acids
Sam QH, Ling H, Yew WS, Tan Z, Ravikumar S, Chang MW, Chai LYA.
International Journal of Molecular Sciences 22(12): 6453 (2021)

Future trends in synthetic biology in Asia
Mao N, Aggarwal N, Poh CL, Cho BK, Kondo A, Liu C, Yew WS, Chang MW
Advanced Genetics 2: e1003 (2021)

Biosynthesis of commodity chemicals from oil palm empty fruit bunch lignin
Lo T-M, Hwang IY, Cho H-S, Fedora RE, Chng SH, Choi WJ, and Chang MW
Frontiers in Microbiology. 12: 663642 (2021)

Searching for unifying laws of general adaptation syndrome: Comment on “Dynamic and thermodynamic models of adaptation” by Gorban et al.
Selvarajoo K
Physics of Life Reviews 37: 97-99 (2021)

COVID-19 endocrinopathy with hindsight from SARS
Kothandaraman N, Rengaraj A, Xue B, Yew WS, Velan SS, Karnani N, Leow MKS
American Journal of Physiology – Endocrinology and Metabolism. 320(1) 320(1): E139–E150 (2021)

A model-driven approach towards rational microbial bioprocess optimization
Yeoh JW, Jayaraman SSO, Tan SGD, Jayaraman P, Holowko MB, Zhang J, Kang CW, Leo HL, Poh CL
Biotechnology Bioengineering 118(1): 305-318 (2021)

Novel modalities in DNA data storage
Lim CK, Nirantar S, Yew WS, Poh CL
Trends in Biotechnology 39(10): 990-1003 (2021)

Development of a polymer-based antimicrobial coating for efficacious urinary catheter protection
Low JL, Kao P H-N, Tambyah PA, Koh GLE, Ling H, Kline KA, Cheow WS, Leong SSL
Biotechnology Notes, 2: 1-10 (2021)


2020

Ectopic overexpression of a cotton plastidial Na+ transporter GhBASS5 impairs salt tolerance in Arabidopsis via increasing Na+ loading and accumulation
Myo T, Tian B, Zhang Q, Niu S, Liu Z, Shi Y, Cao G, Ling H, Wei F, Shi G
Planta 252(3): 41 (2020)

Next-generation metabolic engineering of non-conventional microbial cell factories for carboxylic acid platform chemicals
Li J, Rong L, Zhao Y, Li S, Zhang C, Xiao D, Foo JL, Yu A
Biotechnology Advances 43: 107605 (2020)

Directed computational evolution of quorum-quenching lactonases from the amidohydrolase superfamily
Go MK, Zhao LN, Xue B, Supekar S, Robinson RC, Fan H, Yew WS
Structure 28(6): 635-642.e3 (2020)

Evolving a thermostable terminal deoxynucleotidyl transferase
Chua JPS, Go MK, Osothprarop T, Mcdonald S, Karabadzhak AG, Yew WS, Peisajovich S, Nirantar S
ACS Synthetic Biology 9(7): 1725-1735 (2020)

Capturing multicellular system designs using synthetic biology open language (SBOL)
Brown B, Bartley B, Beal J, Bird JE, Goñi-Moreno A, McLaughlin JA, Mısırlı G, Roehner N, Skelton DJ, Poh CL, Ofiteru ID, James K, Wipat A
ACS Synthetic Biology 9(9): 2410-2417 (2020)

Bioproduction of enantiopure (R)- and (S)-2-phenylglycinols from styrenes and renewable feedstocks
Sekar BS, Mao J, Lukito BR, Wang Z, Li Z
Advanced Synthesis & Catalysis 363(7): 1892-1903 (2020)

Benzoic acid production via cascade biotransformation and coupled fermentation-biotransformation
Zhou Y, Sekar BS, Wu S, Li Z
Biotechnology and Bioengineering 117(8): 2340-2350 (2020)

Tweak to Treat: Reprograming Bacteria for Cancer Treatment
Sieow BFL, Wun KS, Yong WP, Hwang IY, Chang MW
Trends in Cancer, 7(5): 447-464 (2020)

Systems biology approaches integrated with artificial intelligence for optimized metabolic engineering
Helmy M, Smith D, Selvarajoo K
Metabolic Engineering Communications, 11: e00149 (2020)

Systems Biology Approaches for Understanding Biofilm Response
Selvarajoo K
Quorum Sensing: Microbial Rules of Life 2: 9-29 (2020)

ScatLay: utilizing transcriptome-wide noise for identifying and visualizing differentially expressed genes
Bui TT, Lee D, Selvarajoo K
Scientific Reports 10: 17483 (2020)

Engineering an Alcohol-Forming Fatty Acyl-CoA Reductase for Aldehyde and Hydrocarbon Biosynthesis in Saccharomyces cerevisiae
Foo JL, Rasouliha BH, Susanto AV, Leong SSJ, Chang MW
Frontiers in Bioengineering and Biotechnology. 8: 585935 (2020)

Mechanism-Driven Metabolic Engineering for Bio-Based Production of Free R-Lipoic Acid in Saccharomyces cerevisiae Mitochondria
Chen B, Foo JL, Ling H, Chang MW
Frontiers in Bioengineering and Biotechnology. 8: 965 (2020)

Development of a Proline-Based Selection System for Reliable Genetic Engineering in Chinese Hamster Ovary Cells
Sun T, Kwok WC, Chua KJ, Lo T-M, Potter J, Yew WS, Chesnut JD, Hwang IY, Chang MW
ACS Synthetic Biology 9(7): 1864-1872 (2020)

Genetic biosensor design for natural product biosynthesis in microorganisms
Hossain GS, Saini M, Miyake R, Ling H, Chang MW
Trends in Biotechnology. 38(7): 797-810 (2020)

Engineering probiotics for therapeutic applications: recent examples and translational outlook
Aggarwal N, Breedon A, Davis C, Hwang IY, Chang MW
Current Opinion in Biotechnology. 65: 171-179 (2020)

Attractor concepts to evaluate the transcriptome-wide dynamics guiding anaerobic to aerobic state transition in Escherichia coli
Bui TT, Selvarajoo K
Scientific Reports. 10: 5878 (2020)

Blue light-directed cell migration, aggregation, and patterning
Zhang J, Luo Y, Poh CL
Journal of Molecular Biology 432(10): 3137-3148 (2020)

Toolkit development for cyanogenic and gold biorecovery chassis Chromobacterium violaceum
Liow LT, Go MK, Chang MC, Yew WS
ACS Synthetic Biology. 2020, 9, 4, 953-961 (2020)

Engineering Yarrowia lipolytica towards food waste bioremediation: Production of fatty acid ethyl esters from vegetable cooking oil
Ng TK, Yu AQ, Ling H, Juwono NK, Choi WJ, Leong SS, Chang MW
Journal of Bioscience and Bioengineering. 129(1): 31-40 (2020)


2019

A highly sensitive graphene oxide based label-free capacitive aptasensor for vanillin detection
Mohan HKSV, Chee WK, Li Y, Nayak S, Poh CL, Thean AVY
Materials & Design 186: 108208 (2019)

Characterisation of constitutive promoters from the Anderson library in Chromobacterium violaceum ATCC 12472
Liow LT, Go MK, Yew WS
Engineering Biology 3(3): 57-66 (2019)

Resource partitioning strategies during toxin production in Microcystis aeruginosa revealed by integrative omics analysis
Pan D, Pavagadhi S, Umashakar S, Rai A, Benke PI, Rai M, Saxena G, Gangu V, Swarup S
Algal Research 42: 101582 (2019)

Engineering commensal bacteria to rewire host-microbiome interactions
Hwang IY, Chang MW.
Current Opinion in Biotechnology. 62:116-122 (2019)

Phage-boosted chemotherapy.
Aggarwal N, Hwang IY, Chang MW.
Nature Biomedical Engineering. 3(9):680-681 (2019)

Author Correction: Building a global alliance of biofoundries.
Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Feuvre RL, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS.
Nature Communications. 2019 Jul 11;10(1):3132

A novel synchronization approach using synthetic magnetic Chromobacterium violaceum
Ling H, Chang MW.
Synthetic and Systems Biotechnology. 4(3):130-1 (2019)

Meta-omics-and metabolic modelling-assisted deciphering of human microbiota metabolism
Sieow BF, Nurminen TJ, Ling H, Chang MW.
Biotechnology Journal. 30:1800445 (2019)

Building a global alliance of biofoundries
Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Le Feuvre R, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR, Johnson RA, Keasling JD, Kitney RI, Kondo A, Liu C, Martin VJJ, Menolascina F, Ogino C, Patron NJ, Pavan M, Poh CL, Pretorius IS, Rosser SJ, Scrutton NS, Storch M, Tekotte H, Travnik E, Vickers CE, Yew WS, Yuan Y, Zhao H, Freemont PS.
Nature Communications. 10(1):2040 (2019)

Synthetic genetic circuits for programmable biological functionalities
Xia PF, Ling H, Foo JL, Chang MW.
Biotechnology Advances. 37(6):107393 (2019)

Synthetic biology toolkits for metabolic engineering of Cyanobacteria
Xia PF, Ling H, Foo JL, Chang MW.
Biotechnology Journal. 14(6):1800496 (2019)


2018

An oleaginous yeast platform for renewable 1-butanol synthesis based on a heterologous CoA-dependent pathway and an endogenous pathway
Yu A, Zhao Y, Pang Y, Hu Z, Zhang C, Xiao D, Chang MW, Leong SSJ
Microb Cell Fact 17(1):166 (2018)

Synthetic Enzymology and the Fountain of Youth: Repurposing Biology for Longevity
Lim YP, Go MK, Raida M, Inoue T, Wenk MR, Keasling JD, Chang MW, Yew WS
ACS Omega 3(9):11050-11061 (2018)

Genetically Engineered Bacteria as Vehicles for Localized Delivery of Therapeutics: An Application for Crohn’s Disease
McKay R, Ghodasra M, Schardt K, Quan D, Pottash A, Shang W, Jay S, Payne G, Chang M, March J, Bentley W
Bioengineering & Translational Medicine 3(3) (2018)

Synthetic biology toolkits and applications in Saccharomyces cerevisiae
Chen B, Lee HL, Heng YC, Chua N, Teo WS, Choi WJ, Leong SS, Foo JL, Chang MW
Biotechnology Advances 36(7):1870-1881 (2018)

Immunomodulation as Therapy for Fungal Infection: Are We Closer?
Sam QH, Yew WS, Seneviratne CJ, Chang MW, Chai LYA
Frontiers in Microbiology 9(1612) (2018)

Targeted approaches for in situ gut microbiome manipulation
Lee HL, Shen H, Hwang IY, Ling H, Yew WS, Lee YS, Chang MW
Genes 9:351 (2018)

Synthetic yeast genome reveals its versatility
Foo JL, Chang MW
Nature 557:647-648 (2018)

Engineering microbes for targeted strikes against human pathogens
Hwang IY, Lee HL, Huang JG, Lim YY, Yew WS, Lee YS, Chang MW
Cellular and Molecular Life Sciences 75:2719-2733 (2018)

Anhydrous polymer‐based coating with sustainable controlled release functionality for facile, efficacious impregnation, and delivery of antimicrobial peptides
Lim K, Saravanan R, Chong KKL, Goh SHM, Chua RRY, Tambyah PA, Chang MW, Kline KA, Leong SSJ
Biotechnology and Bioengineering 115:2000-2012 (2018)

Reprogramming probiotic Lactobacillus reuteri as a biosensor for Staphylococcus aureus derived AIP-I detection
Lubkowicz D, Ho CL, Hwang IY, Yew WS, Lee YS, Chang MW
ACS Synthetic Biology 7(5):1229-1237 (2018)

Rewriting the Metabolic Blueprint: Advances in Pathway Diversification in Microorganisms
Hossain GS, Nadarajan SP, Zhang L, Ng TK, Foo JL, Ling H, Choi WJ, Chang MW
Frontiers in Microbiology 9:155 (2018)

Engineered commensal microbes for dietmediated colorectal-cancer chemoprevention
Ho CL, Tan HQ, Chua KJ, Kang A, Lim KH, Ling KL, Yew WS, Lee YS, Thiery JP, Chang MW
Nature Biomedical Engineering 2:27–37 (2018)


2017

One-Pot Enantioselective Synthesis of d-Phenylglycines from Racemic Mandelic Acids, Styrenes, or Biobased l-Phenylalanine via Cascade Biocatalysis
Zhou Y, Wu S, Li Z
Advanced Synthesis and Catalysis 359(24):4305–4316 (2017)

Microscale Bioreactors for in situ characterization of GI epithelial cell physiology
Costello C, Philipson M, Hartmanis L, Kwasnica M, Chen V, Hackam D, Chang MW, Bentley W, March J
Scientific Reports 7:12515 (2017)

Autoinducer sensing microarrays by reporter bacteria encapsulated in hybrid supramolecular-polysaccharide hydrogels
Li P, Dou XO, Müller M, Feng C, Chang MW, Frettlöh M, Schönherr H
Macromolecular Bioscience 1700176:8-16 (2017)

Encapsulation of Autoinducer Sensing Reporter Bacteria in Reinforced Alginate-based Microbeads
Li P, Müller M, Chang MW, Frettlöh M, Schönherr H
ACS Appl Mater Interfaces 9 (27):22321–22331 (2017)

Engineering a riboswitch-based genetic platform for the self-directed evolution of acid-tolerant phenotypes
Pham HL, Wong A, Chua N, Teo WS, Yew WS, and Chang MW
Nature Communications 8:411 (2017)

Isolated Reporter Bacteria in Supramolecular Hydrogel Microwell Arrays
Li P, Dou XQ, Feng C, Müller M, Chang MW, Frettlöh M, Schönherr H
Langmuir 33 (31):7799–7809(2017)

Designer probiotics for the prevention and treatment of human diseases
Chua KJ, Kwok WC, Aggarwal N, Sun T, Chang MW
Current Opinion in Chemical Biology 40:8-16 (2017)

Synthetic gene design using Codon Optimization On-Line (COOL)
Yu K, Ang KS, Lee DY
Methods in Molecular Biology 1472:13-34 (2017)

Engineered probiotic Escherichia coli can eliminate and prevent Pseudomonas aeruginosa infection in animal models
Hwang IY, Koh E, Wong CK, March JC, Bentley WE, Lee YS, Chang MW
Nature Communications 8:15028 (2017)

Applying the Design-Build-Test paradigm in microbiome engineering
Pham HL, Ho CL, Wong A, Lee YS, Chang MW
Current Opinion in Biotechnology 48:85-93 (2017)

The fungal mycobiome and interaction with gut bacteria in the host
Sam QH, Chang MW, Chai LYA
International Journal of Molecular Sciences 18:330-341 (2017)

Microbiome engineering: current applications and its future
Foo JL, Ling H, Lee YS, Chang MW
Biotechnology Journal 12:e1600099 (2017)


2016

Highly regio- and enantioselective multiple oxy- and amino-functionalizations of alkenes by modular cascade biocatalysis
Wu S, Zhou Y, Wang T, Too HP, Wang DIC, Li Z
Nature Communications 7:11917 (2016)

Cascade biocatalysis for sustainable asymmetric synthesis: from biobased I-phenylalanine to high-value chiral chemicals
Zhou Y, Wu S, Li Z
Angewandte Chemie 55:11647-11650 (2016)

Multi-omics data driven analysis establishes reference codon biases for synthetic gene design in microbial and mammalian cells
Ang KS, Kyriakopoulos S., Li W, Lee DY
Methods 102:26-35 (2016)

Codon optimization of Saccharomyces cerevisiae mating factor alpha prepro-leader to improve recombinant protein production in Pichia pastoris
Ahn J, Jang MJ, Ang KS, Lee H, Choi ES, Lee DY
Biotechnology Letters 38:2137-2143 (2016)

A genetic algorithm-based approach for pre-processing metabolomics and lipidomics LC-MS data
Yeo HC, Chung BKS, Chong W, Chin JX, Ang KS, Lakshmanan M, Ho YS, Lee DY
Metabolomics 12: 5 (2016)

Transcriptomics-based strain optimization tool for designing secondary metabolite overproducing strains of Streptomyces coelicolor
Kim M, Yi JS, Lakshmanan M, Lee DY, Kim BG
Biotechnology and Bioengineering 113:651-660 (2016)

Coordinate regulation of metabolite glycosylation and stress hormone biosynthesis by TT8 in Arabidopsis
Rai A, Umashankar S, Rai M, Lim BK, Aow JSB, Swarup S
Plant Physiology 171:2499-2515 (2016)

Flagellin FliC phosphorylation affects type 2 protease secretion and biofilm dispersal in Pseudomonas aeruginosa PAO1
Suriyanarayanan T, Periasamy S, Lin MH, Ishihama Y, Swarup S
PLoS One 11:e0164155 (2016)

Reprogrammable microbial cell-based therapeutics against antibiotic-resistant bacteria
Hwang IY, Koh E, Kim HR, Chang MW
Drug Resistance Updates 27:59-71 (2016)

Anti-virulent disruption of pathogenic biofilms using engineered quorum-quenching lactonases
Tay SB, Chow JY, Go M, Yew WS
Journal of Visualized Experiments 107:e53243 (2016)

Exploiting the biosynthetic potential of type III polyketide synthases
Lim YP, Go M, Yew WS
Molecules 21: 53-61 (2016)

A two-layer gene circuit for decoupling cell growth from metabolite production
Lo T, Chng SH, Teo WS, Cho HS, Chang MW
Cell Systems 3:133-143 (2016)

Metabolic engineering of Saccharomyces cerevisiae for the overproduction of short branched-chain fatty acids
Yu A, Pratomo NK, Foo JL, Leong SSJ, Chang MW
Metabolic Engineering 34:36-43 (2016)

Whole-cell biocatalytic and de novo production of alkanes from free fatty acids in Saccharomyces cerevisiae
Foo JL, Susanto AV, Keasling JD, Leong SSJ, Chang MW
Biotechnology and Bioengineering 114:232-237 (2016)

Genetic engineering of an unconventional yeast for renewable biofuel and biochemical production
Yu AQ, Pratomo N, Ng TK, Ling H, Cho HS, Leong SS, Chang MW
Journal of Visualized Experiments 115:e54371 (2016)

Synthetic biology in Asia: New kid on the block
Chang MW
ACS Synthetic Biology 5: 1182-1183 (2016)

Genome-scale metabolic modeling in silico analysis of lipid accumulating yeast Candida tropicalis for dicarboxylic acid production
Mishra P, Park GY, Lakshmanan M, Lee HS, Lee H, Chang MW, Ching CB, Ahn J, Lee DY
Biotechnology and Bioengineering 113: 1993-2004 (2016)


2015

SynLinker: an integrated system for designing linkers and synthetic fusion proteins
Liu CC, Chin JX, Lee DY
Bioinformatics 22:3700-3702 (2015)

Synthetic fusion protein design and applications
Yu K, Liu CC, Kim BG, Lee DY
Biotechnology Advances 33:155-164 (2015)

Effect of linker flexibility and length on the functionality of a cytotoxic engineered antibody fragment
Klement M, Liu CC, Loo BLW, Choo ABH, Ow DSW, Lee DY
Journal of Biotechnology 199: 90-97 (2015)

In silico model-driven cofactor engineering strategies for improving the overall NADP(H) bioavailability in microbial cell factories
Lakshmanan M, Yu K, Koduru L, Lee DY
Journal of Industrial Microbiology and Biotechnology 42: 1401-1414 (2015)

Epoxide hydrolase-lasalocid A structure provides mechanistic insight into polyether natural product biosynthesis
Wong FT, Jotta K, Chen X, Fang M, Watanabe K, Kim CY
Journal of the American Chemical Society 137: 86-89 (2015)

Combinatorial metabolic engineering of Saccharomyces cerevisiae for terminal alkene production
Chen BB, Lee DY, Chang MW
Metabolic Engineering 31:53-61 (2015)

Bacterial XylRs and synthetic promoters function as genetically encoded xylose biosensors in Saccharomyces cerevisiae
Teo WS, Chang MW
Biotechnology Journal 10:315-322 (2015)

Matrix-immobilized yeast for large-scale production of recombinant human lactoferrin
Ho CL, Hwang IY, Loh K, Chang MW
Med Chem Comm 6:486-491 (2015)

Engineering transcription factors to improve against alkane biofuels in Saccharomyces cerevisiae
Ling H, Pratomo NK, Teo WS, Liu R, Leong SSJ, Chang MW
Biotechnology for Biofuels 8:231-242 (2015)

Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid short- and branched-chain alkyl esters biodiesel
Teo WS, Yu A, Chang MW
Biotechnology for Biofuels 8:177-185 (2015)

Synthetic polyketide enzymology: platform for biosynthesis of antimicrobial polyketides
Go M, Wongsantichon J, Cheung, VWN, Chow JY, Robinson RC, Yew WS
ACS Catalysis 5:4033-4042 (2015)

Engineered strains enhance gold biorecovery from electronic scrap
Natarajan G, Tay SB, Yew WS, Ting YP
Minerals Engineering 75:32-37 (2015)

Engineered Saccharomyces cerevisiae to produce odd chain-length fatty alcohols
Jin Z, Wong A, Foo JL, Ng Joey, Cao YX, Chang MW, Yuan YJ
Biotechnology and Bioengineering 113:842-851 (2015)


2014

Production of fatty acid-derived valuable chemicals in synthetic microbes
Yu AQ, Pratomo Juwono NK, Leong SSJ, Chang MW
Frontiers in Bioengineering and Biotechnology 2:78-89 (2014)

Microbial tolerance engineering toward biochemical production: from lignocellulose to products
Ling H, Teo W, Chen B, Leong SSJ, Chang MW
Current Opinion in Biotechnology 29:99-106 (2014)

Site specific immobilization of a potent antimicrobial peptide onto silicone catheters: evaluation against urinary tract infection pathogens
Mishra B, Basu A, Chua RRY, Saravanan R, Tambyah PA, Ho B, Chang MW, Leong SSJ
Journal of Materials Chemistry 2:1706-1716 (2014)

Therapeutic microbes for infectious disease
Wong CK, Tan MH, Rasouliha B, Hwang IY, Ling H, Poh CL and Chang MW
Methods in Molecular Biology 1151:117-133 (2014)

Identification of polyketide inhibitors targeting 3-dehydroquinate dehydratase in the shikimate pathway of Enterococcus faelis
Cheung VWN, Xue B, Valladares MH, Go M, Tung A, Aguda AH, Robinson RC, Yew WS
PLoS ONE 9:e103598 (2014)

Glycine decarboxylase is an unusual amino acid decarboxylase involved in tumorigenesis
Go M, Zhang WC, Lim B, Yew WS
Biochemistry 53: 947-956 (2014)

Disruption of Biofilm Formation by the Human Pathogen Acinetobacter baumannii using engineered quorum-quenching Lactonases
Chow JY, Yang Y, Tay SB, Chua KL, Yew WS
Antimicrobial Agents and Chemotherapy 58: 1802-1805 (2014)